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Why Use an AAV Expression System?

One of the many benefits of using a AAV expression system is that it is a versatile tool for gene delivery. AAVs are able to infect both dividing and non-dividing cells, making them ideal for a wide range of applications. Additionally, AAVs exhibit a low immunogenicity, meaning that they are unlikely to provoke an immune response. This makes them ideal for use in vivo, where other delivery methods may be rejected by the body. There are many companies available that also provide AAV Packaging Service.

Another key advantage of using an AAV expression system is the high efficiency of transduction. This is particularly important when working with Primary cells or cells from hard-to-transfect tissues. In these cases, other methods of transgene delivery (such as transfection) may be less effective.

Overall, the use of a AAV expression system can offer many advantages over other gene delivery methods. When choosing a method for your experiment, it is important to consider all of the potential benefits and drawbacks in order to select the best option for your needs. 

What are some potential drawbacks of using an AAV Expression System?

One potential drawback of using a AAV expression system is the possibility of insertional mutagenesis. This occurs when the AAV integrates its genome into the host cell's DNA, potentially causing changes in gene expression that could lead to cell death or cancer. This risk can be minimized by choosing a AAV with a low integration rate or by using an episomal plasmid for transgene expression.

Another potential drawback of using a AAV expression system is the possibility of immune response. In some cases, the body may recognize the AAV as foreign and mount an immune response against it. This can lead to inflammation and tissue damage. To avoid this, it is important to use a AAV that is well-characterized and has minimal immunogenicity.